Leica STELLARIS FALCON

FLIM (Fluorescence Lifetime Imaging Microscopy) is a special microscopy technique that uses the measurement of the fluorescence lifetime in each pixel of the image, which allows to obtain information about the microenvironment of cells based on this parameter. This technique is valued for its ability to quantitatively measure changes in the microenvironment, such as ion concentration, pH or the presence of molecules in the cellular environment, allowing a more detailed understanding of cellular processes and their regulation. FLIM microscopes are widely used in science and research in fields such as cell biology, neuroscience or medicine, providing information on the dynamics of interactions and processes within cells based on fluorescence characteristics. Observe dynamic molecular interactions with FLIM-FRET (Förster Resonance Energy Transfer) technology. Use biosensors to identify changes in the metabolic environment and microenvironment.